• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    A method for harvesting algae of the genus Dunaliella from suspensions thereof in brines containing sodium chloride at a concentration of about 3M or above, wherein the algal suspension is contacted with an adsorbent having a hydrophobic surface so as to adsorb the algae thereon, and the adsorbent with the algae adsorbed thereon is separated from the brine. beta -carotene and other useful cell components may be extracted from the adsorbed algae by treatment with a suitable solvent.
    公开号:SU1531851A3
    申请号:SU843793658
    申请日:1984-09-26
    公开日:1989-12-23
    发明作者:Куртис Кертан Сирил；Спук Харви
    申请人:Коммонвелф Сайентифик Энд Индастриал Рисерч Организейшн И Бетатен Лимитед (Фирма)；
    IPC主号:
    专利说明:

    This invention relates to biotechnology and concerns the production of vitamins.
    The purpose of the invention is to improve the quality of the f-carotene and simplify the process.
    The method is carried out as follows.
    The isolation of Dunaliella from suspensions in brine has been demonstrated using brine from a natural salt lake. Such a brine is sufficiently saturated with sodium chloride and is typical of brines from such sources in that it is contaminated with clay, halophilic bacteria, and other undesirable materials. The study showed.
    that the number of cells per unit volume is higher than what is usually found in such natural salt waters, and that such cells are vitally important. Only a small amount of lysed cells and fragments of free lipid and free 13-carotene were present in the sample. Individual intact cells contained an increased amount of β-carotene relative to the chlorophyll concentration in them.
    The cell suspension is passed through the stoppers of a porous adsorbent, made of the hydrophobic fibers of nylon 66, polyLyr, polyacrylate,
    ate
    oo
    00
    ate

    cm
    teflon (by: shtetrafluoroethylene) and glass wool, which was made hydrophobic as a result of the treatment with cyanilane. It has been found that in each case there is a good adsorption of Dunaliella cells and the adsorbed algae cells are retained during the subsequent washing of the tubes with cell-free saturated solution, sodium chloride to remove the occluded clay, bacterial cells and other undesirable materials that are present in the initial suspension. Then, the sodium chloride solution, having a concentration below 1 mol, is percolated through the plugs onto which the algae cells are adsorbed, and it has been established that these cells are easily desorbed from the adsorbent and removed from the system with liquid effluent. The thus-recovered adsorbent plugs were found to be capable of adsorbing more Dunaliella cells from a fresh saline sample and this cycle was repeated many times.
    Adsorption of Dunaliella cells to a hydrophobic adsorbent allows cell concentration to occur and facilitates the extraction of i-carotene contained in them. It has been established that Dunaliella cells, remaining adsorbed on a hydrophobic adsorbent, can be destroyed by a solvent capable of degrading the cell membrane, and that the solvent allows for the extraction of α-carotene, leaving the cellular fragments and insoluble cellular components of the adsorbed hydrophobic surface.
    Solvents for this purpose include, but are not limited to, chlorinated solvents such as methylene chloride, chloroform, carbon tetrachloride and trichlorethylene, and aromatic hydrocarbons or mixtures of aromatic and aliphatic hydrocarbons. Hydrocarbons such as benzene can be used in this pellet , toluene and prom), 1silinee petroleum fractions containing mixtures of aromatic or aliphatic and aromatic hydrocarbons. Aromatic hydrocarbons are used for; and than aliphatic carbon 0
    five
    0
    five
    0
    five
    0
    five
    0
    five
    Hydrogens, since they have a greater ability to dissolve the carotene components. L. h of the subsequent extraction of β-carotene, it is preferred to use solvents with a relatively low boiling point, on the order of 10 ° C below that. A suitable solvent for the extraction of J-Ka-rotin is liquid carbon dioxide and a low boiling point of such a solvent minimizes the tendency for the extracted carotin components to decompose during their separation and isolation.
    In addition to ft-carotene, the soluble component also contains triterpenoids and hypnids. Such components can be easily separated from | 3 -carotene, for example, by fractional crystallization and / or distribution between the solvent and they can be separated for other purposes, for example, as a chemical raw material.
    The continuous OCKOJUCHs remaining after the extraction of y-carotene can be desorbed with the adsorbent by washing the latter with a solution of sodium chloride chloride sp1 with water according to the described method, and the adsorbent thus regenerated can be pennpKyjnipoBaTbCH for further use.
    Cell debris, which is a protein material, can be extracted using a suitable method and used, for example, as feed for animal feed or protein supplement, stubs as a protein source for other uses.
    The water cell component, mainly glycerin, can also be extracted from the washing fluid.
    Example 1. Glass wool is melted and made hydrophobic as a result of plating for 10 minutes in a 10% (v / v) solution of dichlorodimethylsilane in hexane. Then the treated glass wool is washed five times with distilled water. A column 15 cm long and 7 cm in outer diameter carefully stuffed 90 g of the glass wool treated with the above method and 1.5 l of Dunaliella Salyina suspension to a saturated solution of ethereal sodium, salt 10 cells / ml, etc. 1) cherch the following about
    5153
    speed of 1.3 l / min. The liquids are then allowed to drain from the column and 700 ml of methylene chloride are passed through it. Waste pschy methylene chloride is collected and analyzed for content. B-carotene. It was found that the effluent contains 77% of L-carotene present in the cells contained in the initial solution.
    The glass wool is then reactivated by passing 1.6 liters of distilled water through the column for the purpose of desorption and rapid removal of the remaining cell fragments. Reactivated glass wool is reused in an identical experiment with the following sample of the same Dunaliella cell suspension. There were almost identical results.
    Example 2. Magnetite (10 g) passing through a 20-mesh sieve is silanized with a 10% (v / v) solution of dichloromethylsilane in hexane for 20 minutes, then washed with water and dried at 110 ° C Silanized magnetite is mixed with 50 ml of a suspension containing 2x10 cells / ml Dunaliella in a saturated solution of sodium chloride for 10 minutes. The silanized magnetite is then removed from the solution using a permanent magnet. The chains adsorbed on magnetite are lysed by contacting magnetite with methylene chloride, after which methylene chloride is separated and analyzed for its B-carotene content. It was established that 87% of the carotene present in the initial suspension of Dunaliella cells was extracted with methylene chloride.
    Silanized magnetite is reacted by washing five times with 10 ml portions of water. The experiment is then repeated using 70% B-carotene, which is present in the Dunaliella cell suspension, using reactivated magnetite and extracted with methylene chloride.
    Example 3. Polyester fiber (1 g) was carefully loaded onto a column 20 cm long and 1.5 cm outer diameter. Dunaliella Salina cell suspension (2x10 cells / ml) in a saturated sodium chloride solution was passed through the column at a rate of 80 ml / min. Then the fiber is washed
    0
    516
    20 MJI on the sodium chloride solution and allow the liquid to drain from the column. The fiber is then transferred to the Scraplet Extractor and extracted with carbon tetrachloride. Analysis of the extracted extract showed that 2.1 mg of p-carotene was obtained from Dunaliella cells, which were adsorbed on
    Q (Polyester fiber.
    Example 4. Repetition method of example 3, except that the polyester fiber was replaced with 1 g of nylon fiber. An analysis of the extract revealed that 0.5 mg of B-carotene was extracted.
    Example 5. Polytetrafluoroethylene fiber (1.5 g) is activated by heating to 316 ° C in 98% sulfuric acid and a sufficient amount of nitric acid is added to decolorize the mixture. The fiber is removed, washed with water, dried and used in accordance with the procedure of Example 3. p-carotene (0.4 mg per g of fiber) is extracted by extraction with carbon tetrachloride.
    Example 6. Acrylic fiber (1 g) was treated according to the procedure described in Example 3. 0.5 mg of B-carotene was isolated.
    Example 7. Anthra1; it (12.5 g) is ground so that it passes through a 120 mesh sieve (BSS) and is then mixed for 30 minutes with a saturated solution of sodium chloride (250 ml) containing 2x10 cells / ml Dunaliella Salina. Then the mixer is stopped, the mixture is allowed to settle before the top layer of liquid is salted and the contents are filtered through a plug of loosely packed glass wool. Both the precipitated material and the material remaining on the filter are washed with an exemplary solution of sodium chloride (20 ml). and the washings are discarded. The filter is transferred to the same container as the precipitate, and the combined solids are washed with successive portions of methylene chloride until the washing liquids lose the color created by the carotene present. A solution of methylene chloride is analyzed, it is found that it contains 0.7 mg | 3-carotene per gram of anthracite used.
    0
    0
    five
    0
    Example 8. Graphite (12.5 g) was used instead of anthracite and the procedure described in Example 7 was repeated. A solution of methylene chloride was analyzed and it was found that it contained 0.6 mg of R-carotene per gram of graphite.
    Example 9. According to the procedure described in Example 7, chalcopyrite (12.5 g) was used and as a result, 0.6 mg of B-carotene per gram of chalcopyrite was extracted.
    Example 10. Sphalerite (12.5 g was applied as described in Example 7. and as a result, 0.25 mg ft-carotene per gram sphalerite was extracted.
    Example 11. Pyrolusite (12.5 was used according to the procedure described in Example 7, and 0.25 mg of carotene per gram of pyrolusite was recovered.
    Example 12 Rutile (12.5 g) was used as described in Example 7. As a result, 0.2 mg of β-carotene per gram of rutile was recovered.
    Example 13. Ilmenite (12.5 g was used according to the procedure described in Example 7, and as a result, 0.4 mg of B-carotene per gram of ilmenite was obtained
    Etc. and measure 14. Magnetite (12.5 g was used according to the procedure described in Example 7. Except that a permanent magnet was used to separate magnetite instead of a glass wool filter. As a result, 3.3 mg of β-carotene per gram of magnetite.
    Example 15. 50g of gematite with a particle size of about 120 mesh (BSS) is dried at 105 ° C for one hour and then treated with 1.4 g of dichloromethylsilane in 290 ml of petroleum ether for 12 hours at 20 C. Then the petroleum ether is removed on the suction filter and the treated hamacite is dried at 105 ° C for 1 h. 10 g of the dried treated hamatite is added to one liter of Dunaliella Salina cell suspension in a saturated solution of sodium chloride. The hamatite is then removed by decantation and magnetism and extracted with 100 ml of dichloromethane. 81% of p-carotene was isolated, based on the amount contained in the initial suspension P5 of Dunaliella Salina cells.
    Example 16. 50 g of magnetite are treated with 2.5 ml of aminopropyltri0
    five
    0
    five
    0
    five
    0
    five
    ethoxysilane and 2.5 ml of acetic acid in 250 ml of water for 10 minutes, then the liquid is partially removed by suction filtration, leaving important magnetite, which is heated at 105 ° C for 5 c to complete the reaction of the silane with magnetite. The treated magnetite is washed with 100 ml of ethanol and dried at 105 ° C. for one hour. 10 g of the sample is taken from the resulting batch and used according to the procedure described in Example 15. As a result, 84% -carotene is recovered.
    Example 17. 1 kg of magnetite with a particle size of about 100 mesh (BSS) is treated with 2 liters of a 1% solution of dichlorodimethylsilane in petroleum ether for 2 hours at room temperature. Then, the petroleum ether solution is removed by filtration under vacuum and the treated magnetite is dried at 105 ° C for 1 hour. Prepare a suspension of Dunaliella Salina in a saturated solution of sodium chloride, the first of which contains 0.5 mg / l, and the second 5.0 mg / l L - carotene. Portions of dried magnetite are mixed with 3 liter aliquots of Dunaliella Salina suspensions for 5 minutes. Then the magnetite is removed from the salt solutions using a magnet and extracted with 100 ml of hexane. The hexane solution is analyzed colorimetrically for the content of 3-carotene as a result of the absorption of light at a wavelength of 460 nm.
    In tab. Figure 1 shows the magnitude of the B-carotene recovery when using the indicated concentrations of 0-carotene in suspensions and the indicated amounts of magnetite.
    Table 1
    Example 18. 500 g of dry magnetosite with a part size of 120 mega (BSS) are treated with 1 liter of 1% aqueous solution of dichlorodimethylsilane in petroleum ether at ambient temperature for 3 hours. The treated magnetite is separated from the solvent by decantation and merging with a magnet , dried for 0.5 h and then demagnetized. The pieces remaining in the mixture are broken. Each of the seven 3-liter aliquots of brine containing Dunaliella (with salt copies) is mixed with 60 g of silanized magnetite and mixed thoroughly for 5 minutes. Then the magnetite is collected using a magnet and dried on a filter operating under vacuum.
    The seven magnetite samples thus obtained, containing the adsorbed Dunaliella cells, are combined and evenly distributed over the surface of thin glass wool, which is then placed on one blade of a U-shaped vessel for pressure operation. Then this vessel is inverted and 500 ml of liquid carbon dioxide (under pressure AOOO kPa and 8 ° C) are introduced and this solvent is allowed to re-percolate through glass wool by repeated inversion of the vessel. Liquid carbon dioxide is then removed and replaced with a fresh aliquot of liquid. Three 500 ml aliquots of liquid carbon dioxide are used. Evaporation of carbon dioxide gives respectively 0.36, 0.38 and 0.15 g of a red oily liquid, which crystallizes with cooling to -5 ° C with the formation of 23 mg of crystalline 3-carotene.
    Example 19. Glass wool was subjected to silanization and imparted hydrophobic properties by immersion for 10 minutes in a 10% (v / v) solution of dichlorodimethylsilane (A156 silane, Union Carbide) in hexane. The treated glass wool is then washed five times with distilled water. A 15 cm long column with an external diameter of 7 cm is carefully filled with 90 g of glass wool thus treated and 1.5 suspensions of Dunaliella Salina cells in a saturated solution of sodium chloride and containing 1.0 x 10 cells / ml are passed through this column with a space velocity of 1 3 l / min The collected effluent (1.5 L) contains 1.1 x 10 cells / ml, which indicates that 89% of the cells introduced into the column are adsorbed on glass wool.
    Then, a saturated solution of sodium chloride (1.5 l), which does not contain any current, is passed through the column at the same volumetric rate and the resulting stream is also collected. It is established that the stream contains a certain number of bacterial cells and part, clay, and, both of them are contained in the initial suspension, but the content of Dunaliella Salina cells in it is less than 10 cells / ml, which indicates that
    f. essentially no cells adsorbed on glass wool.
    Then, 1 liter of a 0.5 M solution of sodium chloride in distilled water is passed through the column;
    5 necks of cells, while the resulting stream is collected.
    The effluent was found to contain 1.3 X 10 Dunaliella Salina / MJi cells, which corresponds to 97%
    Q cells that are adsorbed onto glass wool.
    Then the glass wool is regenerated with a HELP OF 1.6 liter of dissociated water; F1 through the column
     so as to wash away any trash
    cells or fragments of cells. The regenerated glass vagu is again used for a similar experiment with another sample of the same Dunaliella suspension, and identical results are obtained.
    Example 20. A series of experiments were carried out according to the procedure described in Example 19, then with the exception that silanized glass wool was replaced with organic polymer fibers. In each case, the adsorption of Dunalliella cells is good, and
    0 adsorbed algal cells are retained when a saturated solution of sodium chloride is passed through the column in order to remove the absorbed parts of the pigment.
    5 clay and bacterial cells. Essentially, all KJICTKTI adsorbed algae are desorbed when they are passed through column P, 5 M radar 0
    five
    sodium chloride thief. The results of the experiments are given in table. 2
    Tabliya2
    Polytetrafluoroethylene activated before use by heating to 316 C in 98% sulfuric acid with enough nitric acid added to make the mixture V colorless. The fiber is then removed, washed with water and dried before use.
    As in Example 19, the fibers are regenerated by passing water through the column, and then used again in identical experiments with other samples of the same Dunaliella suspension, which resulted in essentially similar results.
    Example 21. Magnetite (10 g), pumped through a 120 mesh sieve (ESS), hole diameter of the O screen, 123 mm, is subjected to silanization using a 10% (v / v) solution of dichlorodimethylsilane in hexane for 20 minutes then washed with water and dried at 110 ° C. Silanized magnetite is mixed with 50 ml of a suspension containing 2x10 cells per ml of Dunaliella Salina in a saturated solution of x-sodium sodium for 10 minutes after which silanized magnetite is removed from the solution with constant magnet.
    0
    five
    0
    five
    five
    0
    The solution was found to contain 3x10 cells / ml, indicating that 85% of the cells originally contained in the suspension were bio-adsorbed onto magnetite.
    Then, the magnetite is suspended in 50 ml of a bulk cell-free solution of sodium chloride, stirred for 10 minutes, and removed as before. The solution was found to contain 1.2x10 cells / ml, which indicates that essentially the cells are not desorbed from magnetite.
    I
    Then the magnetite ne1) is mixed with 30 ml of 0.5 M rasporov sodium chloride cell free, for 10 min and removed, as it was done before, the resulting solution contains 2.6 x 10 Dunaliel-1a / ml, which corresponds to 92 % desorbed cells previously adsorbed on magnetite.
    The silanized 1.g1 magnetite is regenerated by five times washing with portions of fl 10 ml of water. Then the experiment was repeated, as a result of which it was determined that 837 cells i initially contained in the suspension were adsorbed on magnetite, and 93% of these adsorbed cells were desorbed with a 0.5 M solution of sodium chloride.
    Example 22. The procedure of Example 21 is followed with the exception that magnetite is charged for iron. It has been established that 82% of Dunaliella cells are adsorbed on a red iron and that the adsorbed cells are not desorbed with the pure sodium chloride solution. After mixing the loaded red iron with a 0.5 N solution of sodium chloride, 95% of the adsorbed cells were desorbed.
    Example 23. The procedure of Example 21 is followed, with the silanized magnetite being replaced by a series of hydrophobic minerals, since these minerals were non-magnetic, they were removed from the solution during the precipitate, decantation and decantation of the upper layer; through the filter containing a not very tightly packed gasket and glass wool. Reza 111, tats iriredeny in table. 3,
    13 t a b
    faces
    1851
    using a 0.5 M solution of sodium chloride.
    The invention makes it possible to obtain p-carotene. In fact, it does not contain undesirable carotenoids, lipids, etc., the source of which are halobacteria, and the invention will simplify the process by eliminating complicated filtering or centrifuging operations.
    From tab. 3 that the good adsorption of Dunaliella cells takes place from a saturated solution of sodium chloride and that an extremely high desorption is achieved when using the formula
    The method of producing α-carotene from a suspension of algae of the Dunaliella genus in a sodium chloride solution with a tie concentration of less than 3 M. comprising separating the sodium chloride solution from
    algae, extraction of algae with a solvent and removal of the latter from the extract, which is different from the fact that, in order to improve the quality of PI-carotene and simplify the process, the separation of the sodium chloride solution from the algae is carried out by contacting the suspension with a particulate or fibrous hydrophobic adsorbent.
    权利要求:
    Claims (1)
    [1]
    Claim
    A method for producing β-carotene from a suspension of algae of the genus Dunaliella in a solution of sodium chloride with a concentration of at least 3 M. Including separation of the sodium chloride solution from the algae, extraction of the algae with a solvent and removal of the latter from the extract, characterized in that, in order to improve the quality of | b -carotene and simplification of the process, the separation of the sodium chloride solution from algae is carried out in contact with the saturated sodium chloride solution and that extremely high desorption is achieved by using a suspension with a corpuscular or fibrous hydrophobic adsorbent.
    类似技术:
    公开号 | 公开日 | 专利标题
    SU1531851A3|1989-12-23|Method of obtaining beta-carotene from suspension of algae dunaliella in solution on sodium chloride with concentration nott below 3 m
    US3803033A|1974-04-09|Process for removal of organic contaminants from a fluid stream
    CA2166717A1|1995-01-19|A method for the production of adsorption material
    CN101367572A|2009-02-18|Method for removing saralytic saxitoxin
    JP4098984B2|2008-06-11|Method for recovering pigment from algae cultures
    IL44879A|1977-10-31|Biochemical syntheses and degradations using entrapped enzymes
    HU195532B|1988-05-30|Process for yielding weeds
    JPH06336501A|1994-12-06|Process for recovering cyclodextrin
    AU758066B2|2003-03-13|A method for recovering pigments from algal cultures
    Bouyssiere et al.2003|3.2 Sample preparation techniques for elemental speciation studies
    JPH11178595A|1999-07-06|Separation of pigment from microbial cell
    Tsuruta et al.2018|Removal and recovery of gold | by biosorption and biomineralization using Pseudomonas saccharophila
    US2072089A|1937-03-02|Method of obtaining and isolating finely dispersed substances, possessing an electrical charge, from strongly diluted solutions
    RU2049129C1|1995-11-27|Method for recovery of gold from ore materials
    JPH07100765B2|1995-11-01|Extraction method of blue pigment from cyanobacteria
    CN109174064A|2019-01-11|The preparation of bentonite-moringa seeds albumen composite adsorbing material and its application method
    SU1120016A1|1984-10-23|Method of reclaminating used vegetable oil in vegetable roasting process
    CN112973618A|2021-06-18|Layered metal sulfide adsorbent, preparation method thereof and method for selectively enriching lead ions from heavy metal wastewater
    CN110294489A|2019-10-01|The processing method of rare-earth chloride solution
    US4826624A|1989-05-02|Dispersing liquid hydrocarbon into a second liquid
    CN107497408A|2017-12-22|New polycyclic aromatic hydrocarbon compounds（PAHs）The preparation method and application of adsorbent
    JPH0751055B2|1995-06-05|Method for extracting blue pigment from cyanobacteria
    Suzuki et al.1996|A simple procedure for large‐scale purification of 9‐cis β‐carotene from Dunaliella bardawil
    CN107670650A|2018-02-09|A kind of quality rebuilding method of porous adsorbing material after absorption malachite green
    CN108654582A|2018-10-16|A kind of biological adsorption agent prepared using Thallus Laminariae | extract
    同族专利:
    公开号 | 公开日
    DE3268859D1|1986-03-13|
    JPS58501654A|1983-10-06|
    FI70923C|1986-10-27|
    EP0089983A1|1983-10-05|
    EP0089983B1|1986-01-29|
    NO832052L|1983-06-07|
    FI832038A0|1983-06-07|
    DK260083A|1983-06-07|
    FI70923B|1986-07-18|
    BR8207915A|1983-09-13|
    WO1983001257A1|1983-04-14|
    EP0089983A4|1984-03-01|
    FI832038L|1983-06-07|
    RO89653A|1986-06-30|
    US4554390A|1985-11-19|
    DK260083D0|1983-06-07|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    RU2497945C2|2012-03-20|2013-11-10|Федеральное государственное бюджетное учреждение науки Институт клеточного и внутриклеточного симбиоза Уральского отделения Российской академии наук|STRAIN OF UNICELLULAR ALGAE Dunaliella salina IPPAS D-295-PRODUCER OF BIOLOGICALLY ACTIVE SUBSTANCES HAVING ANTIOXIDANT ACTIVITY|US2620334A|1949-12-08|1952-12-02|Kraft Foods Co|Process of extraction from irish moss|
    US3195271A|1962-05-18|1965-07-20|Clarence G Golueke|Process for culturing and recovering algae and carageenin|
    AU487018B2|1973-08-16|1976-02-19|Bayonet Pty. Ltd.|Extraction of caroteniferous materials from algae|
    CA1063955A|1975-01-06|1979-10-09|Julian E. Blanch|Removal of trace heavy metal contaminants from algae and the carrageenan contained therein|
    IL49726A|1976-06-06|1979-09-30|Yeda Res & Dev|Production of glycerol from algae|
    US4199895A|1977-05-25|1980-04-29|Yeda Research And Development Co. Ltd.|Production of glycerol, carotenes and algae meal|
    JPS54137073A|1978-04-17|1979-10-24|Daicel Chem Ind Ltd|Antistatic treatment of plastic molded article having abrasion resistant coating|
    FR2453199B1|1979-04-06|1982-01-08|Inst Francais Du Petrole|
    IL57712A|1979-07-03|1984-02-29|Yissum Res Dev Co|Cultivation of halophilic algae of the dunaliella species for the production of fuel-like product|US4910912A|1985-12-24|1990-03-27|Lowrey Iii O Preston|Aquaculture in nonconvective solar ponds|
    AU611869B2|1986-10-17|1991-06-27|Western Biotechnology Ltd.|A method for improving recovery by centrifugation of microorganisms from suspension in aqueous solutions|
    US4871551A|1988-02-08|1989-10-03|Microbio Resources, Inc.|Pigmentation supplements for animal feed compositions|
    CA1339250C|1988-03-21|1997-08-12|Richard Calvin Ebersole|Method and apparatus for collecting and detecting microorganisms|
    US4958460A|1988-05-09|1990-09-25|Algae Farms|Method of growing and harvesting microorganisms|
    US20080175953A1|1995-06-07|2008-07-24|Martek Biosciences Corporation|Process for the Heterotrophic Production of Microbial Products with High Concentrations of Omega-3 Highly Unsaturated Fatty Acids|
    US5340742A|1988-09-07|1994-08-23|Omegatech Inc.|Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids|
    US20060094089A1|1988-09-07|2006-05-04|Martek Biosciences Corporation|Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids|
    JP2646160B2|1991-03-08|1997-08-25|美穂 田中|Feed for pet birds and its manufacturing method|
    EP0531639B1|1991-07-18|1999-09-01|F. Hoffmann-La Roche Ag|Tocopherol cyclase|
    US20040180865A1|1993-06-04|2004-09-16|Jeffry W. Kreamer|Aspirin and vitamin and/or trace element compositions for the amelioration and treatment of vascular disease|
    CN1047773C|1995-08-31|1999-12-29|徐贵义|Method for preparation of natural carotene|
    US6000551A|1996-12-20|1999-12-14|Eastman Chemical Company|Method for rupturing microalgae cells|
    WO1998028404A1|1996-12-20|1998-07-02|Eastman Chemical Company|Method for deep bed filtration of microalgae|
    US5776349A|1996-12-20|1998-07-07|Eastman Chemical Company|Method for dewatering microalgae with a jameson cell|
    US5910254A|1996-12-20|1999-06-08|Eastman Chemical Company|Method for dewatering microalgae with a bubble column|
    US5951875A|1996-12-20|1999-09-14|Eastman Chemical Company|Adsorptive bubble separation methods and systems for dewatering suspensions of microalgae and extracting components therefrom|
    US5807023A|1997-03-21|1998-09-15|Krenzler; Leo M.|Artificial reef with corrodible iron inserts|
    US20020082459A1|1997-05-28|2002-06-27|Bailey David T.|High purity beta-carotene and process for obtaining same|
    ES2262503T3|1999-08-09|2006-12-01|Beta Carotene Investments Limited|A METHOD TO RECOVER PIGMENTS FROM ALGAE CROPS.|
    IN189919B|1999-11-11|2003-05-10|Proalgen Biotech Ltd|
    MX345559B|2000-01-28|2017-02-03|Dsm Ip Assets B V |Enhanced production of lipids containing polyenoic fatty acids by high density cultures of eukaryotic microbes in fermentors.|
    AU2003902794A0|2003-06-03|2003-06-19|Agresearch Limited|Improvements in grass endophytes|
    CN100457890C|2005-09-01|2009-02-04|宁波大学|Novel method of effective separating manifold micro algae germplasm|
    TWI580778B|2007-06-19|2017-05-01|再生海藻能源公司|Process for microalgae conditioning and concentration|
    DE112009001548T5|2008-06-24|2011-04-21|Innovative Creations Business Modules Pvt. Ltd.|Medium and method for the cultivation of algae|
    US20100170150A1|2009-01-02|2010-07-08|Walsh Jr William Arthur|Method and Systems for Solar-Greenhouse Production and Harvesting of Algae, Desalinationof Water and Extraction of Carbon Dioxide from Flue Gas via Controlled and Variable Gas Atomization|
    CA2754769A1|2009-03-09|2010-09-16|Univenture, Inc.|Method and apparatus for separating particles from a liquid|
    US20110165662A1|2009-07-13|2011-07-07|Inventure Chemical, Inc.|Method for harvesting microalgae suspended in an aqueous solution using a hydrophobic chemical|
    DE102009039554A1|2009-09-07|2011-03-10|Phytolutions Gmbh|A method of harvesting algae from an algal suspension, first, second and third algal suspension concentrates and first, second and third nutrient liquid filtrate|
    US8518132B2|2009-12-11|2013-08-27|Albert C. Rettenmaier|Methods of algae harvesting utilizing a filtering substance and uses therefor|
    US8450111B2|2010-03-02|2013-05-28|Streamline Automation, Llc|Lipid extraction from microalgae using a single ionic liquid|
    US8722375B2|2010-03-05|2014-05-13|Raytheon Company|Algal cell lysis and lipid extraction using electromagnetic radiation-excitable metallic nanoparticles|
    US8399239B2|2010-03-26|2013-03-19|Solix Biofuels, Inc.|Compositions and methods for continuous harvesting of suspension growth cultures|
    US8303818B2|2010-06-24|2012-11-06|Streamline Automation, Llc|Method and apparatus using an active ionic liquid for algae biofuel harvest and extraction|
    US8828705B1|2010-11-18|2014-09-09|Iowa State University Research Foundation, Inc.|Magnetic mesoporous material for the sequestration of algae|
    WO2012153174A2|2011-05-06|2012-11-15|Ariel-University Research And Development Company, Ltd.|Wastewater treatment method and device|
    DE102011087137A1|2011-11-25|2013-05-29|Fim Biotech Gmbh|A method for separating microorganisms from an aqueous phase and an apparatus for carrying out this method|
    US8938909B2|2012-11-26|2015-01-27|National Taiwan Ocean University|Process of rapid isolating Monostroma latissimum filamentous bodies for mass-scale breeding|
    US9556088B2|2012-11-30|2017-01-31|Iowa State University Research Foundation, Inc.|Adsorbent catalytic nanoparticles and methods of using the same|
    US9567265B2|2012-11-30|2017-02-14|Iowa State University Research Foundation, Inc.|Catalysts and methods of using the same|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    AUPF109381|1981-10-07|
    [返回顶部]